Acclaim 120 C18 Reversed-Phase LC Column

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High Performance Reversed-Phase Columns for the Separation of Small Molecules

The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.

  • Ultrapure silica with high-density bonding for low silanol activity

  • Very high efficiencies for maximum resolution

  • Reproducible manufacturing practices for reproducible column-to-column performance

  • LC/MS compatible

Acclaim 120 C18 columns have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and available in 3 and 5 μm particles sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120 Å. Acclaim 120 C18 is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC columns section for more details).  All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.

Wide Range of Applications

The Acclaim 120 C18 can be used for the many diverse reversed-phase C18 separations. Its rugged, reproducible, and reliable characteristics make it appropriate for pharmaceutical, environmental, food and other industrial chromatographic separations. The Acclaim Catalog has many examples of the use of this column.

Acclaim 120 HPLC column production begins with ultrapure silica, which results in symmetrical peak shapes due to ultra-low metal content. Next, a unique bonding chemistry produces a very well-ordered and high-density silica surface coverage, which results in highly predictable reversed-phase separations, unaffected by secondary retention mechanisms.


Acclaim 120 Silica-Based Reversed-Phase Columns for Small Molecule Analysis Specifications
Particle shapeSpherical
Particle size distribution (40/90)1.2
Total carbon contentC18: 18%
C8: 11.2%
Carbon surface coverageC18: 3.2 μmol/m2
C8: 3.7 μmol/m2
Pore volume0.9 mL/g
Average pore diameter120 Å
Surface area300 m2/g
pH Range2–8
Temperature<60 °C
End-cappedYes
Metal impurity (Na, Fe, Al)<10.0 ppm Total Metal
<3.0 ppm Individual Metal
USP listingC18: L1
C8: L7
Starting materialUltrapure silica
Particle size3, 5 µ


Antioxidants in Edible Oils by AOAC Method 983.15.

 

Caffeine analogs.

 

Assay of vanilla extract.

 

Alkaloids in bitter orange extract.


Standard particle sizes are 3 and 5 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm diameters; standard lengths are 50, 100, and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Micro precolumn and nano precolumn cartridges are available in several formats. Guard columns in both 2.0 and 4.3 mm sizes packed with 5 μm particles are recommended to protect analytical columns. Columns for fast LC are listed in the RSLC section.

Guard Columns
Acclaim 120, C18, 5µm Guard Cartridges, (2.1 x 10mm) 2 ea; requires holder 069580069689
Acclaim 120 C18, 5µm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580071981
Acclaim 120, C18, 5 µm Guard Cartridges, (4.6 x 10 mm), (use V-2 holder)069695
Acclaim SST Guard Cartridge Holder V-2069580
Acclaim Guard Kit (Holder and coupler) V-2069707
Guard to Analytical Column Coupler V-2074188
Analytical Columns
Acclaim 120, C18, 3 µm Analytical (2.1 x 50 mm)059128
Acclaim 120, C18, 3 µm Analytical (2.1 x 100 mm)059129
Acclaim 120, C18, 3 µm Analytical (2.1 x 150 mm)059130
Acclaim 120, C18, 3µm Analytical (2.1 x 250mm)076187
Acclaim 120, C18, 3µm Analytical (3.0 x 100mm)076186
Acclaim 120, C18, 3 µm Analyitcal (3.0 x 150 mm)063691
Acclaim 120,C18, 3µm, Analytical, (3.0 x 250mm)070077
Acclaim 120, C18, 3 µm Analytical (4.6 x 50 mm)059131
Acclaim 120, C18, 3 µm Analytical (4.6 x 100 mm)059132
Acclaim 120, C18, 3 µm Analytical (4.6 x 150 mm)059133
Acclaim 120, C18, 5 µm Analytical (2.1 x 50 mm)059142
Acclaim 120, C18, 5 µm Analytical (2.1 x 100 mm)059143
Acclaim 120, C18, 5 µm Analytical (2.1 x 150 mm)059144
Acclaim 120, C18, 5 µm Analytical (2.1 x 250 mm)059145
Acclaim 120, C18, 5 µm Analytical (4.6 x 50 mm)059146
Acclaim 120, C18, 5 µm Analytical (4.6 x 100 mm)059147
Acclaim 120, C18, 5 µm Analytical (4.6 x 250 mm)059149
Acclaim 120, C18, 5 µm Analytical (4.6 x 150 mm)059148
Acclaim RSLC 120 C18 Analytical Columns
Acclaim RSLC 120, C18, 3 µm Analytical (3.0 x 33 mm)066272
Acclaim 120 C18, 3µm Analytical, (3.0 x 50mm)068971
Acclaim RSLC 120, C18, 3 µm Analytical (3.0 x 75 mm)066273
Acclaim 120, C18, 3 µm Analyitcal (3.0 x 150 mm)063691
Acclaim RSLC 120, C18, 2.2µm Analytical (2.1 x 30 mm)071400
Acclaim RSLC 120, C18, 2.2µm Analytical (2.1 x 50 mm)068981
Acclaim RSLC 120, C18, 2.2µm Analytical (2.1 x 100 mm)068982
Acclaim RSLC 120, C18, 2.2µm Analytical, (2.1 x 150mm)071399
Acclaim RSLC 120, C18, 2.2µm Analytical (2.1 x 250mm)074812
Acclaim RSLC 120, C18, 2.2 µm Analytical, (3.0 x 30mm)071606
Acclaim RSLC 120, C18, 2.2 µm Analytical, (3.0 x 50mm)071605
Acclaim RSLC 120, C18, 2.2 µm Analytical, (3.0 x 100mm)071604
Micro and Nano Columns
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper164567
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 15 cm, nanoViper164568
300 µm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper164716
Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm i.d. × 15 cm, nanoViper164571
1.0 mm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper164717
Acclaim PepMap100, C18, 3 μm, 100 Å, 1 mm i.d. × 15 cm, nanoViper164572
Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper164567
75 µm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper164708
Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 25 cm160326
300 µm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper164716
Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm i.d. × 15 cm, nanoViper164571
Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 25 cm160300
1.0 mm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper164717
1.0 mm i.d. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å, nanoViper164711
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm160287
Micro and Nano Precolumns
Acclaim 120, C18, 5 µm, 120 Å, 300 µm i.d. x 5 mm, 5 Cartridges162326
Acclaim PepMap100, C18, 5 µm, 100 Å, 500 µm i.d. x 5 mm, 5 Cartridges160446
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 5 mm, 5 Cartridges160434
Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 15 mm, 5 Cartridges160438