Acclaim C30 Reversed-Phase Column with High Structural Selectivity

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Acclaim C30 Reversed-phase LC Columns
The Acclaim® C30 is designed to provide high shape selectivity for separation of hydrophobic structurally related isomers and unique selectivity complementary to other reversed-phase columns (e.g. C18). Key benefits include:

• High shape selectivity
• Unique selectivity complementary to other reversed-phase columns
• Compatibility with highly aqueous mobile phases
• High-quality columns with low column bleed, high efficiency, and rugged packing

Acclaim C30 columns are available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 200 Å. It is based on covalent modification of C30 alkyl silane on high-purity, spherical, porous silica gel. The combination of advanced surface modification technology and careful matching of C30 alkyl chain with the pore size of the silica substrate results in a unique chemistry ideal for separating hydrophobic structurally related isomers.


Applications
The Acclaim C30 is a reversed-phase HPLC column and can be used in a wide range of applications including foods and beverages, chemical, environmental, pharmaceutical, academia, etc. The Acclaim C30 columns exhibit high shape selectivity suited to separation of hydrophobic, long chain, structurally related isomers (e.g. carotenoids, steroids, etc). They are also fully compatible with various aqueous buffers, useful for a broad application range of analytes(e.g. water-soluble vitamins, organic acids, etc) and more flexibility in method development. The Acclaim C30 column is a viable alternative to normal-phase columns for lipid analysis. The Acclaim C30 columns can be used for LC/MS applications.


Acclaim C30:  Specifications
Column ChemistryProprietary alkyl C30
End-cappedYes
Particle Size3 and 5 µm
Average pore diameter200 Å
Surface area200 m2/g
pH range2–8
Temperature<60 °C
Starting materialUltrapure silica
Particle shapeSpherical



Separation of glucocorticosteroids.


Separation of omega fatty acids


Separation of water-soluble vitamins


Analysis of soybean lecithin and egg lecithin


The National Institute of Standards and Technology (NIST) Standard Reference Material (SRM) 869a is useful for characterizing liquid chromatographic (LC) column selectivity for separation of PAHs. 

These separations demonstrate the different complementary selectivity of the Acclaim C30 column shows to other reversed-phase columns.

Comparison of C18 and C30 in the separation of peanut oil.

Separation of various oils using the Acclaim C30 column.

Phospholipid separation using the Acclaim C30 column.

The Acclaim C30 column is compatible with aqueous operating conditions.


Analytical Columns
Acclaim C30, 3µm, Analytical, 4.6 x 150mm075723
Acclaim C30, 3µm, Analytical,(4.6 x 100mm)078660
Acclaim C30, 3µm, Analytical,(4.6 x 50mm)078661
Acclaim C30, 3µm, Analytical, (3.0x250mm)075726
Acclaim C30, 3µm, Analytical, (3.0 x 150mm)075724
Acclaim C30, 3µm, Analytical,(3.0 x 100mm)078662
Acclaim C30, 3µm, Analytical,(3.0 x 50mm)078663
Acclaim C30, 3µm, Analytical,(2.1 x 250mm)078664
Acclaim C30, 3µm, Analytical, (2.1x150mm)075725
Acclaim C30, 3µm, Analytical,(2.1 x 100mm)078665
Acclaim C30, 3µm, Analytical,(2.1 x 50mm)078666
Acclaim C30, 5µm, Analytical, (4.6 x 250mm)075718
Acclaim C30, 5µm, Analytical, (4.6 x 150mm)075719
Guard Columns
Acclaim C30, 5µm, Guard (2.1 x10mm), 2ea, (use V-2 Holder)075722
Acclaim C30, 5µm, Guard (3.0 x10mm), 2ea, (use V-2 Holder)075721
Acclaim C30, 5µm, Guard (4.6 x10mm), 2 ea, (use V-2 Holder)075720