Acclaim 120 C8 Reversed-Phase LC Column

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High Performance Reversed-Phase Columns for the Separation of Small Molecules

Acclaim® 120 C8 reversed-phase columns feature a densely-bonded monolayer of octyldimethylsiloxane on a highly pure, spherical silica substrate with a 120 Å pore structure. The columns are a well-characterized line of LC/MS-compatible C8 phases with very high surface coverage and very low metal content. These columns provide exceptional performance for a variety of applications in the pharmaceutical, nutraceutical, food, and life sciences.

  • Ultrapure silica with high-density bonding for symmetrical peak shapes

  • High hydrophobicity, low polarity phases for predictable reversed-phase separations

Acclaim 120 C8 column have high surface coverage, resulting in high-capacity columns.  They are stable between pH 2 and 8 and are available in 3 and 5µm particle sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120Å. Acclaim 120 C8 is also available in 2.2µm RSLC format (see Acclaim RApid Separation LC Columns section for more details). All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.


Acclaim 120 Silica-Based Reversed-Phase Columns for Small Molecule Analysis  Specifications
Particle shapeSpherical
Particle size distribution (40/90)1.2
Total carbon contentC18: 18%
C8: 11.2%
Carbon surface coverageC18: 3.2 μmol/m2
C8: 3.7 μmol/m2
Pore volume0.9 mL/g
Average pore diameter120 Å
Surface area300 m2/g
pH Range2–8
Temperature<60 °C
End-cappedYes
Metal impurity (Na, Fe, Al)<10.0 ppm Total Metal
<3.0 ppm Individual Metal
USP listingC18: L1
C8: L7
Starting materialUltrapure silica
Particle size3, 5 µ


Isocratic separation of six cardiac antiarrhythmic drugs (Beta-Blockers).

 

Loperamide in anti-diarrhea tablets.

 

Fluticasone in serum.

 

Carbamate insecticides in EPA 531.1 with MS detection.


Analytical Columns
Acclaim 120, C8, 3 µm Analytical (2.1 x 50 mm)059122
Acclaim 120, C8, 3 µm Analytical (2.1 x 100 mm)059123
Acclaim 120, C8, 3 µm Analytical (2.1 x 150 mm)059124
Acclaim 120, C8, 3µm Analytical (2.1 x 250mm)076185
Acclaim 120, C8, 3µm Analytical (3.0 x 150 mm)068970
Acclaim 120, C8, 3µm Analytical (3.0 x 100mm)076184
Acclaim 120,C8, 3 µm, Analytical,(3.0 x 250 mm)070078
Acclaim 120, C8, 3 µm Analytical (4.6 x 50 mm)059125
Acclaim 120, C8, 3 µm Analytical (4.6 x 100 mm)059126
Acclaim 120, C8, 3 µm Analytical (4.6 x 150 mm)059127
Acclaim 120, C8, 5 µm Analytical (2.1 x 50 mm)059134
Acclaim 120, C8, 5 µm Analytical (2.1 x 100 mm)059135
Acclaim 120, C8, 5 µm Analytical (2.1 x 150 mm)059136
Acclaim 120, C8, 5 µm Analytical (2.1 x 250 mm)059137
Acclaim 120, C8, 5 µm Analytical (4.6 x 50 mm)059138
Acclaim 120, C8, 5 µm Analytical (4.6 x 100 mm)059139
Acclaim 120, C8, 5 µm Analytical (4.6 x 150 mm)059140
Acclaim 120, C8, 5 µm Analytical (4.6 x 250 mm)059141
Guard Columns
Acclaim 120, C8, 5 µm Guard Cartridges, (4.6 x 10 mm), 2 ea (use V-2 Holder)069696
Acclaim 120 C8, 5µm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580071979
Acclaim 120, C8, 5µm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580069688
Acclaim SST Guard Cartridge Holder V-2069580
Acclaim Guard Kit (Holder and coupler) V-2069707
Guard to Analytical Column Coupler V-2074188
Acclaim RSLC 120 C8 Analytical Columns
Acclaim RSLC C8, 2.2 µm Analytical (2.1 x 30 mm)072614
Acclaim RSLC 120, C8, 2.2µm, 2.1 x 50 mm072615
Acclaim RSLC 120, C8, 2.2µm, 2.1 x 100 mm072616
Acclaim RSLC 120, C8, 2.2µm, 2.1 x 150 mm072617
Acclaim RSLC 120, C8, 2.2µm Analytical (2.1 x 250mm)074811
Acclaim RSLC 120, C8, 2.2µm, 3 x 30mm072618
Acclaim RSLC 120, C8, 2.2µm, 3 x 50mm072619
Acclaim RSLC 120, C8, 2.2µm, 3 x 100mm072620
Micro and Nano Columns
Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 5 cm161555
75 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164706
300 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164722
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 5 cm161547
Acclaim PepMap100, C8, 3 µm, 100 Å1.0 mm i.d. x 5 cm160240
1.0 mm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164723
Acclaim PepMap100, C8, 5 µm, 100 Å, 75 µm i.d. x 5 cm161555
75 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164706
75 µm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164706
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 5 cm161547
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 15 cm161545
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 5 cm161539
1.0 mm i.d. x 15 cm, packed with Acclaim PepMap100 C8, 3 µm, 100Å, nanoViper164723
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm161180
Micro and Nano Precolumns
Acclaim PepMap100, C8, 5 µm, 100 Å, 300 µm i.d. x 5 mm, 5 Cartridges161194
Acclaim PepMap100, C8, 5 µm, 100 Å, 500 µm i.d. x 5 mm, 5 Cartridges161192
Acclaim PepMap100, C8, 5 µm, 100 Å, 500 µm i.d. x 5 mm, 5 Cartridges161192
Acclaim 120, C8, 5 µm, 120 Å, 800 µm i.d. x 5 mm, 5 Cartridges162263
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 5 mm, 5 Cartridges161189
Acclaim PepMap100, C8, 5 µm, 100 Å, 1.0 mm i.d. x 15 mm, 5 Cartridges161190