Acclaim 120 C18 Reversed-Phase LC Column

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High Performance Reversed-Phase Columns for the Separation of Small Molecules

The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.

· Ultrapure silica with high-density bonding for low silanol activity
· Very high efficiencies for maximum resolution
· Reproducible manufacturing practices for reproducible column-to-column performance
· LC/MS compatible

Acclaim 120 C18 columns have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and available in 3 and 5 μm particles sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120 Å. Acclaim 120 C18 is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC columns section for more details).  All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.


Wide Range of Applications


The Acclaim 120 C18 can be used for the many diverse reversed-phase C18 separations. Its rugged, reproducible, and reliable characteristics make it appropriate for pharmaceutical, environmental, food and other industrial chromatographic separations. The Acclaim Catalog has many examples of the use of this column.

Acclaim 120 HPLC column production begins with ultrapure silica, which results in symmetrical peak shapes due to ultra-low metal content. Next, a unique bonding chemistry produces a very well-ordered and high-density silica surface coverage, which results in highly predictable reversed-phase separations, unaffected by secondary retention mechanisms.

Acclaim 120 Silica-Based Reversed-Phase Columns for Small Molecule Analysis Specifications

Particle shape

Spherical

Particle size distribution (40/90)

1.2

Total carbon content

C18: 18%
C8: 11.2%

Carbon surface coverage

C18: 3.2 μmol/m2
C8: 3.7 μmol/m2

Pore volume

0.9 mL/g

Average pore diameter

120 Å

Surface area

300 m2/g

pH Range

2–8

Temperature

<60 °C

End-capped

Yes

Metal impurity (Na, Fe, Al)

<10.0 ppm Total Metal
<3.0 ppm Individual Metal

USP listing

C18: L1
C8: L7

Starting material

Ultrapure silica

Particle size

3, 5 µ


Antioxidants in Edible Oils by AOAC Method 983.15.


Caffeine analogs.


Assay of vanilla extract.


Alkaloids in bitter orange extract.


Standard particle sizes are 3 and 5 μm. Analytical columns are available in 2.1, 3.0, and 4.6 mm diameters; standard lengths are 50, 100, and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Micro precolumn and nano precolumn cartridges are available in several formats. Guard columns in both 2.0 and 4.3 mm sizes packed with 5 μm particles are recommended to protect analytical columns. Columns for fast LC are listed in the RSLC section.


Guard Columns

Acclaim 120, C18, 5碌m Guard Cartridges, (2.1 x 10mm) 2 ea; requires holder 069580

069689

Acclaim 120 C18, 5碌m Guard Cartridges (3 x 10mm), 2ea, requires holder 069580

071981

Acclaim 120, C18, 5 碌m Guard Cartridges, (4.6 x 10 mm), (use V-2 holder)

069695

Acclaim SST Guard Cartridge Holder V-2

069580

Acclaim Guard Kit (Holder and coupler) V-2

069707

Guard to Analytical Column Coupler V-2

074188


Analytical Columns

Acclaim 120, C18, 3 碌m Analytical (2.1 x 50 mm)

059128

Acclaim 120, C18, 3 碌m Analytical (2.1 x 100 mm)

059129

Acclaim 120, C18, 3 碌m Analytical (2.1 x 150 mm)

059130

Acclaim 120, C18, 3 碌m Analytical (2.1 x 250mm)

076187

Acclaim 120, C18, 3 碌m Analytical (3.0 x 100mm)

076186

Acclaim 120, C18, 3 碌m Analyitcal (3.0 x 150 mm)

063691

Acclaim 120, C18, 3 碌m, Analytical (3.0 x 250mm)

070077

Acclaim 120, C18, 3 碌m Analytical (4.6 x 50 mm)

059131

Acclaim 120, C18, 3 碌m Analytical (4.6 x 100 mm)

059132

Acclaim 120, C18, 3 碌m Analytical (4.6 x 150 mm)

059133

Acclaim 120, C18, 5 碌m Analytical (2.1 x 50 mm)

059142

Acclaim 120, C18, 5 碌m Analytical (2.1 x 100 mm)

059143

Acclaim 120, C18, 5 碌m Analytical (2.1 x 150 mm)

059144

Acclaim 120, C18, 5 碌m Analytical (2.1 x 250 mm)

059145

Acclaim 120, C18, 5 碌m Analytical (4.6 x 50 mm)

059146

Acclaim 120, C18, 5 碌m Analytical (4.6 x 100 mm)

059147

Acclaim 120, C18, 5 碌m Analytical (4.6 x 250 mm)

059149

Acclaim 120, C18, 5 碌m Analytical (4.6 x 150 mm)

059148


Acclaim RSLC 120 C18 Analytical Columns

Acclaim RSLC 120, C18, 3 碌m Analytical (3.0 x 33 mm)

066272

Acclaim 120 C18, 3碌m Analytical, (3.0 x 50mm)

068971

Acclaim RSLC 120, C18, 3 碌m Analytical (3.0 x 75 mm)

066273

Acclaim 120, C18, 3 碌m Analyitcal (3.0 x 150 mm)

063691

Acclaim RSLC 120, C18, 2.2碌m Analytical (2.1 x 30 mm)

071400

Acclaim RSLC 120, C18, 2.2碌m Analytical (2.1 x 50 mm)

068981

Acclaim RSLC 120, C18, 2.2碌m Analytical (2.1 x 100 mm)

068982

Acclaim RSLC 120, C18, 2.2碌m Analytical, (2.1 x 150mm)

071399

Acclaim RSLC 120, C18, 2.2碌m Analytical (2.1 x 250mm)

074812

Acclaim RSLC 120, C18, 2.2 碌m Analytical, (3.0 x 30mm)

071606

Acclaim RSLC 120, C18, 2.2 碌m Analytical, (3.0 x 50mm)

071605

Acclaim RSLC 120, C18, 2.2 碌m Analytical, (3.0 x 100mm)

071604


Micro and Nano Columns

Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper 

164567

Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 15 cm, nanoViper

164568

300 µm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper

164716

Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm i.d. × 15 cm, nanoViper

164571

1.0 mm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper

164717

Acclaim PepMap100, C18, 3 μm, 100 Å, 1 mm i.d. × 15 cm, nanoViper

164572

Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper 

164567

75 µm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper

164708

Acclaim PepMap100, C18, 5 µm, 100 Å, 75 µm i.d. x 25 cm

160326

300 µm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper

164716

Acclaim PepMap100, C18, 3 μm, 100 Å, 300 μm i.d. × 15 cm, nanoViper

164571

Acclaim PepMap100, C18, 5 µm, 100 Å, 300 µm i.d. x 25 cm

160300

1.0 mm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper

164717

1.0 mm i.d. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å, nanoViper

164711

Acclaim PepMap100, C18, 5 µm, 100 Å, 1.0 mm i.d. x 25 cm

160287



Micro and Nano Precolumns

Acclaim 120, C18, 5 碌m, 120 脜, 300 碌m i.d. x 5 mm, 5 Cartridges

162326

Acclaim PepMap100, C18, 5 碌m, 100 脜, 500 碌m i.d. x 5 mm, 5 Cartridges

160446

Acclaim PepMap100, C18, 5 碌m, 100 脜, 1.0 mm i.d. x 5 mm, 5 Cartridges

160434

Acclaim PepMap100, C18, 5 碌m, 100 脜, 1.0 mm i.d. x 15 mm, 5 Cartridges

160438