OmniPac PAX-500 Multimode Column

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The OmniPac® PAX-500 column is a macroporous, low-capacity, solvent-compatible column designed to separate anionic and neutral species in a single run. This column combines both anion-exchange and polymeric reversed-phase retention mechanisms in a single column.

  • Good for the simultaneous separation of neutral and anionic species

  • Unique selectivity for polar and ionic organic analytes

  • Effective for biological samples such as purines, pyrimidines, and vitamins

  • Good for drugs and pharmaceuticals

The multimode separation mechanism of the OmniPac PAX-500 is achieved by coating a macroporous, hydrophobic core for reversed-phase retention, with an anion-exchange latex bead layer for anion-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. The anion-exchange selectivity is provided by an anion-exchange MicroBead latex that coats the hydrophobic core. Since the anion-exchange layer is identical to that of the OmniPac PAX-100, the two columns exhibit similar ion-exchange selectivity. As a result, the OmniPac PAX-100 can be used to develop the ion-exchange separation and the method can then be transferred to the OmniPac PAX-500 for refinement to include the reversed-phase separation. The OmniPac PAX-500 column is 100% solvent compatible and should always be run with at least 1% organic solvent in the mobile phase to maintain column life.

The OmniPac PAX-500 may be used for the separation of compounds such as alcohols, alkanolamines, antihistamines, anti-inflammatory agents, aromatic acids, inorganic acids, peptides, purines and pyrimidines, sulfonamides, sulfonated anionic surfactants, and water-soluble vitamins.


OmniPac PAX-500 Multimode Column  Specifications
DimensionsOmniPac PAX-500 Analytical Column:
4 × 250 mm
OmniPac PAX-500 Guard Column:
4 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Recommended operating temperatureAmbient
Typical operating conditions1.0 mL/min with at least 1% organic solvent
Recommended flow rate: 1.0 mL/min
Mobile phase compatibility100% compatible with common organic solvents.
Recommended eluents: solvent for reversed-phase, anion, such as Cl or OH for anion-exchange.
Resin composition8.5-µm-diameter ethylvinylbenzene/divinylbenzene macroporous substrate (55% cross-linked) agglomerated with 60-nm MicroBead alkanolquaternary amine-functionalized latex (4% cross-linked).
Anion-exchange capacityApproximately 40 µeq/column (4 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.


Gradient Separation with Two-Dimensional Chromatography on a Single Column.


Analytical Columns
OmniPac PAX-500 Analytical Column (4 x 250 mm)042152
Guard Columns
OmniPac PAX-500 Guard Column (4 x 50 mm)042153