OmniPac PCX-500 Mixed-Mode Column

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The OmniPac® PCX-500 column is a macroporous, high-capacity, solvent-compatible column designed to separate cationic and neutral species in a single run. This column combines cation-exchange and polymeric reversed-phase retention mechanisms in a single column.

  • Good for the simultaneous separation of neutral and cationic species

  • Acid and base compatible, pH 0–14

  • 100% solvent compatible

  • Not as effective as the CS12A for inorganic cations

The multimode separation mechanism is achieved by coating a macroporous hydrophobic core for reversed-phase retention with a cation-exchange latex bead layer for cation-exchange retention. The macroporous structure provides an accessible hydrophobic core where reversed-phase retention occurs. The cation-exchange selectivity is provided by a cation-exchange MicroBead layer that coats the hydrophobic core. Since the cation-exchange layer is identical to that of the OmniPac PCX-100, the two columns exhibit similar ion-exchange selectivity. As a result, the OmniPac PXC-100 can be used to develop the ion-exchange separation, and the method can then be transferred to the OmniPac PXC-500 for further refinement to include solvent for the reversed-phase separation. The OmniPac PCX-500 is 100% solvent compatible and should always be run with at least 1% organic solvent in the mobile phase to maintain column life.

The OmniPac PCX-500 may be used for the separation of compounds such as adrenergics, alcohols, anilines, antidepressants, anti-inflammatory agents, plating bath brighteners, cephalosporins, stains and dyes, herbicides, nucleotides and nucleosides, peptides, sulfonamides, and water-soluble vitamins.

OmniPac PCX-500 Mixed-Mode Column  Specifications
DimensionsOmniPac PCX-500 Analytical Column:
4 × 250 mm
OmniPac PCX-500 Guard Column:
4 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Recommended operating temperatureAmbient
Typical operating conditions1.0 mL/min with at least 1% organic solvent
Recommended flow rate: 1.0 mL/min
Mobile phase compatibility100% compatible with common organic solvents. Recommended eluents: 1% solvent. Acid to maintain weak bases in the cationic form, salt (e.g., Na+ for cation exchange).
Resin composition8.5-µm-diameter ethylvinylbenzene/divinylbenzene macroporous substrate (55% cross-linked) agglomerated with 200-nm MicroBead sulfonic acid-functionalized latex (5% cross-linked).
Anion-exchange capacity120 µeq/column (4 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.

Gradient Separation of N-Containing Compounds Using the OmniPac PCX-500 Column


Cation-Exchange/IPAD S-Containing Amino Acids


Homocysteine in Plasma (Heart Cut) PCX-500 Cation Exchange with IPAD Detection.

Analytical Columns
OmniPac PCX-500 Analytical Column (4 x 250 mm)042191
Guard Columns
OmniPac PCX-500 Guard Column (4 x 50 mm)042195